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Author:Salminen, Kalle
Title:Hiilipastaelektrodit elektrokemiluminesenssissa
Carbon paste electrodes in electrogenerated chemiluminescence
Publication type:Master's thesis
Publication year:2013
Pages:iv + 100      Language:   fin
Department/School:Kemian laitos
Main subject:Epäorgaaninen kemia   (Kem-35)
Supervisor:Kulmala, Sakari
Instructor:
OEVS:
Electronic archive copy is available via Aalto Thesis Database.
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Location:P1 Ark Aalto  1913   | Archive
Keywords:HECL
hot electron induced electrogenerated chemiluminescence
carbon paste electrodes
elektrokemiluminesenssi
kuumien elektronien tuottama elektrokemiluminesenssi
hiilipastaelektrodit
Abstract (eng): In the literature section electro generated chemiluminescence is discussed in detail.
Major emphasis is given for hot electron-induced electro generated chemiluminescence.
In this phenomenon electrically formed intermediates react to form excited molecules.
This in turn produces light which can be used for analytical purposes.
Often this is accomplished by labelling analytically interesting molecules with luminophores having long exited-state lifetimes.
Method offers low detection limits and excellent specificity.
Instruments based on this phenomenon are widely used in clinical laboratories, especially in the field of immunoassays.

The objective in the experimental part of this work was to study manually manufactured carbon paste electrodes and their usability in hot electron induced electro generated chemiluminescence.
Compared to previously used electrodes carbon paste electrodes are both easier and cheaper to manufacture.
Experiments were done by using Ru(bpy)32+ and Tb(III)-chelate as luminophores.
Detection limits for these luminophores with different coreactants were obtained.
In addition, factors affecting the manufacturing process of electrodes were studied in order to achieve the best detection limits possible.
Abstract (fin): Työn kirjallisuusosiossa käsitellään elektrokemiluminesenssia, pääpainon ollessa kuumien elektronien tuottamassa elektrokemiluminesenssissa.
Kyseisessä ilmiössä sähkön avulla tuotetut intermediaatit reagoivat kemiallisesti elektrodin lähellä muodostaen virittyneen molekyylin.
Virittyneestä tilasta emittoituvaa valoa voidaan hyödyntää analyyttisessa kemiassa esimerkiksi merkitsemällä analyyttisesti mielenkiintoinen molekyyli luminoforilla.
Menetelmän etuihin lukeutuvat pieni toteamisraja, spesifisyys ja vaatimattomat laitevaatimukset yhdessä laajan lineaarisen alueen kanssa.
Elektrokemiluminesenssiin perustuvien instrumenttien käyttäminen on melko suosittua kliinisissä laboratorioissa esimerkiksi leima-ainesovelluksissa bioanalytiikassa.

Diplomityön kokeellisessa osassa tutkittiin itse valmistettujen kertakäyttöisten hiilipastaelektrodien soveltuvuutta Ru(bpy)32+:n ja Tb(lll)-kelaatin katodiseen elektrokemiluminesenssiin erilaisissa koejärjestelyissä.
Verrattuna elektrokemiluminesenssissa perinteisesti käytettyihin elektrodimateriaaleihin, ovat hiilipastaelektrodit halvempia sekä nopeampia valmistaa. toteamisrajojen lisäksi tutkittiin hiilielektrodien valmistamiseen liittyvien parametrien vaikutusta toistettavuuteen sekä mittasignaalin suuruuteen.
ED:2013-08-07
INSSI record number: 47028
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