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Author:Kyrklund, Dan Mikael
Title:CS-proteiinin biotekninen valmistaminen ja sen hyödyntäminen malariarokotteissa
Biotechnical production of CS-protein and its use in malaria vaccines
Publication type:Master's thesis
Publication year:2010
Pages:vii + 70 s. + [11]      Language:   fin
Department/School:Biotekniikan ja kemian tekniikan laitos
Main subject:Bioprosessitekniikka   (Kem-70)
Supervisor:Leisola, Matti
Instructor:Turunen, Ossi
OEVS:
Electronic archive copy is available via Aalto Thesis Database.
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Location:P1 Ark Aalto  3451   | Archive
Keywords:Plasmodium falsiparum
CPS
GST-fusion protein
Plasmodium falsiparum
CSP
GST-fuusioproteiini
Abstract (eng): The aim of this study was the biotechnical production and characterization of N- and C-terminal domains of CS -surface protein from malaria parasite.
The produced proteins were aimed to be used in further studies focusing on the structural analysis of the protein and its binding characteristics to other proteins.

The literature part of this study goes through malaria infections that are caused by Plasmodium -protozoa and the challenges that vaccine technology faces in relation to it.
The main focus of the literature part is in malaria parasite's CS-protein which has been proven to have a role in the birth of malaria infection.
C- and N-terminal domains of CS-protein have a major role in the parasite's life cycle in the human body.
Due to its important role in the beginning of the infection the CS-protein has been a target in the development of malaria vaccines.
Vaccine development against the Plasmodium-parasite is however very challenging.
The immune response that it creates is very complicated and poorly understood.

In the research part, the CS-protein's N-terminal domain was constructed by PCR and cloned into the pGEX-5X-1-vector.
The protein was produced in Escherichia coli -bacteria and purified by utilizing the ability of GST-fusion protein to bind to Glutathione Sepharose.
The production of CS-protein's C-terminal domain was not successful and thus, the research part concentrated only on the N-terminal domain.
The transfer of large concentrations of the protein to the inclusion bodies caused a lot of problems in the production and purification steps.
The quantity of the produced and purified protein remained smaller than planned.
This might cause problems in the crystallizing and the structural analysis of the protein in further studies.
Nevertheless, we were able to produce protein for further experiments.
Abstract (fin): Työn tavoitteena oli bioteknisesti valmistaa malariaparasiitin CS-pintaproteiinin N- ja C -terminaalista osaa.
Valmistettuja proteiineja käytetään myöhemmissä jatkotutkimuksissa proteiinin rakenteen selvittämiseen sekä sitoutumisominaisuuksien tutkimiseen.

Työn kirjallisuusosassa käsitellään Plasmodium-alkueläimen aiheuttamaa malariainfektiota ja siihen liittyviä rokoteteknologian haasteita.
Kirjallisuusosassa keskitytään malariaparasiitin CS -pintaproteiiniin, jonka on osoitettu olevan osallisena malariainfektion synnyssä.
CS-proteiinin N- ja C-terminaalisella päällä on merkittävä rooli parasiitin kiertokulussa ihmiselimistössä.
CS -proteiinin merkittävän aseman takia sitä on pyritty hyödyntämään kehitteillä olevissa malariarokotteissa.
Rokotteen kehittäminen Plasmodium-parasiittia vastaan on kuitenkin erittäin haastavaa, sillä parasiittitaudit nostavat monimutkaisen immuunivasteen, jonka toiminnan tunteminen on edelleen puutteellista.

Tutkimusosassa CS-proteiinin N-terminaalinen osa valmistettiin PCR-reaktion avulla ja liitettiin osaksi pGEX-5X-l-vektoria.
Proteiini tuotettiin Escherichia coli-bakteerituottokannassa ja puhdistettiin GST-fuusioproteiinia hyödyntämällä.
CS-proteiinin C-terminaalisen osan tuottamisessa ei onnistuttu, joten tutkimusosassa keskityttiin pelkästään N-terminaaliseen osaan.
Tuotetun proteiinin siirtyminen suuressa maan n inkluusiokappaleisiin aiheutti paljon ongelmia proteiinin tuotossa ja puhdistuksessa.
Koska proteiinimäärä jäi tavoiteltua pienemmäksi, voi proteiinin kiteyttäminen ja rakenteen selvittäminen olla jatkotutkimuksissa hankalaa.
Proteiinia saatiin kuitenkin tuotettua muita jatkotutkimuksia varten.
ED:2010-07-05
INSSI record number: 39806
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