search query: @author Suoranta, Laura / total: 2
results-list
reference: 1 / 2
« previous | next »
Author:Suoranta, Laura
Title:Development of cryopreservation methods for selected plant and algae cultures
Kryopreservaatiomenetelmien kehittäminen valikoiduille kasvisolu- ja kudosviljelmille
Publication type:Master's thesis
Publication year:2012
Pages:v + 89 s. + liitt. 19      Language:   eng
Department/School:Kemian laitos
Main subject:Biokemia ja mikrobiologia   (Kem-30)
Supervisor:Nordström, Katrina
Instructor:Häkkinen, Suvi ; Rischer, Heiko
OEVS:
Electronic archive copy is available via Aalto Thesis Database.
Instructions
close

Reading digital theses in the closed network of the Aalto University Harald Herlin Learning Centre

In the closed network of Learning Centre you can read digital and digitized theses not available in the open network.

The Learning Centre contact details and opening hours: https://learningcentre.aalto.fi/en/harald-herlin-learning-centre/

You can read theses on the Learning Centre customer computers, which are available on all floors.

Logging on to the customer computers

  • Aalto University staff members log on to the customer computer using the Aalto username and password.
  • Other customers log on using a shared username and password.

Opening a thesis

  • On the desktop of the customer computers, you will find an icon titled:

    Aalto Thesis Database

  • Click on the icon to search for and open the thesis you are looking for from Aaltodoc database. You can find the thesis file by clicking the link on the OEV or OEVS field.

Reading the thesis

  • You can either print the thesis or read it on the customer computer screen.
  • You cannot save the thesis file on a flash drive or email it.
  • You cannot copy text or images from the file.
  • You cannot edit the file.

Printing the thesis

  • You can print the thesis for your personal study or research use.
  • Aalto University students and staff members may print black-and-white prints on the PrintingPoint devices when using the computer with personal Aalto username and password. Color printing is possible using the printer u90203-psc3, which is located near the customer service. Color printing is subject to a charge to Aalto University students and staff members.
  • Other customers can use the printer u90203-psc3. All printing is subject to a charge to non-University members.
Location:P1 Ark Aalto  1884   | Archive
Keywords:cryopreservation
plant biotechnology
kryopreservaatio
kasvibiotekniikka
Abstract (eng): Cryopreservation can be defined as the storage of a living organism, or a part thereof, in temperatures colder than -130°C (typically in liquid nitrogen, -196 °C).
In these temperatures, all cellular processes are switched off, which enables the storage of cells and tissues for theoretically an unlimited period of time.
Plant cell and tissue cultures are sources of valuable substances, for which they are extensively studied in biotechnology.
Maintenance of these cultures, however, is labour-intensive, carrying also the risk of contamination and genetic drift.
Therefore, cryopreservation is of remarkable benefit in handling of these cultures, although the freezing methods must be determined for each culture individually.

The aim of this Master's thesis was to develop cryopreservation methods for seven cultures studied in the Plant Biotechnology Group of the Technical Research Center of Finland (VTT): cell suspension cultures of Catharanthus roseus, Nicotiana tabacum and Vaccinium vitis-idaea, hairy root cultures of Nicotiana tabacum, and microalgal suspension cultures of Chlorella pyrenoidosa, Scenedesmus obliquus and Nitzschia microcephalia.
The microalgae were cryopreserved with relatively good regrowth frequencies, using glycerol beads and controlled rate cooling.
Suspension cells of V. vitisidaea were recovered after encapsulation-dehydration with moderate frequencies, as were the hairy roots of N. tabacum preserved with controlled rate cooling.
C. roseus indicated high post-thaw viabilities but produced no regrowth, while the experiments with N. tabacum suspension cells showed no signs of cryopreservation success.
Abstract (fin): Kryopreservaatio voidaan määritellä elävän organismin, tai sen osan, säilömisenä alle -130 °C:ssa (tyypillisesti nestetypessä, -196 °C).
Näissä lämpötiloissa kaikki solun prosessit pysähtyvät, mikä mahdollistaa solujen ja kudosten säilömisen teoriassa rajoittamattomaksi ajaksi.
Kasvisoluja -kudosviljelmät tuottavat lukuisia arvokkaita yhdisteitä, joiden vuoksi ne ovat laajan kiinnostuksen kohteena biotekniikan alalla.

Viljelmien ylläpito on kuitenkin työlästä, minkä lisäksi niiden käsittelyyn liittyy esimerkiksi kontaminaatioiden ja geneettisten muutosten riski.
Näin ollen kryopreservaation edut kasvisolu- ja -kudosviljelmien käsittelyssä ovat ilmeisiä, vaikka menetelmät joudutaan räätälöimään jokaiselle viljelmälle yksilöllisesti.

Tämän diplomityön tarkoituksena oli kehittää kryopreservaatiomenetelmät seitsemälle viljelmälle Valtion teknillisen tutkimuskeskuksen (VTT) Kasvibiotekniikan tutkimusryhmässä: Catharanthus roseus -, Nicotiana tabacum - ja Vaccinium vitis-idaea -kasvien solususpensioviljelmille, N. tabacum -kasvin karvajuuriviljelmille sekä mikrolevien Chlorella pyrenoidosa, Scenedesmus obliquus ja Nitzschia microcephalia suspensioviljelmille.

Mikroleväviljelmät kryopreservoitiin glyserolihelmien ja kaksivaiheisen pakastuksen avulla, joilla saavutettiin verrattain nopea toipuminen.
Hitaampi toipuminen havaittiin V. vitis-idaea -soluilla enkapsulaatiodehydraatiomenetelmällä, samoin kuin kaksivaiheisella pakastuksella preservoiduilla N. tabacum -karvajuurilla.
C. roseus -solut indikoivat korkeita elävyyksiä sulatuksen jälkeen, mutta kasvua ei havaittu.
N. tabacum -suspensio puolestaan ei osoittanut lainkaan merkkejä onnistumisesta.
ED:2012-04-23
INSSI record number: 44264
+ add basket
« previous | next »
INSSI