search query: @keyword knockout / total: 2
reference: 1 / 2
« previous | next »
Author:Rajaraman, Sitaram
Title:Computational analysis of heart transcriptome in genetically modified rats
Publication type:Master's thesis
Publication year:2012
Pages:52      Language:   eng
Department/School:BIT-tutkimuskeskus
Main subject:Informaatiotekniikka   (T-61)
Supervisor:Lähdesmäki, Harri ; Aurell, Erik
Instructor:Kivelä, Riikka
OEVS:
Electronic archive copy is available via Aalto Thesis Database.
Instructions

Reading digital theses in the closed network of the Aalto University Harald Herlin Learning Centre

In the closed network of Learning Centre you can read digital and digitized theses not available in the open network.

The Learning Centre contact details and opening hours: https://learningcentre.aalto.fi/en/harald-herlin-learning-centre/

You can read theses on the Learning Centre customer computers, which are available on all floors.

Logging on to the customer computers

  • Aalto University staff members log on to the customer computer using the Aalto username and password.
  • Other customers log on using a shared username and password.

Opening a thesis

  • On the desktop of the customer computers, you will find an icon titled:

    Aalto Thesis Database

  • Click on the icon to search for and open the thesis you are looking for from Aaltodoc database. You can find the thesis file by clicking the link on the OEV or OEVS field.

Reading the thesis

  • You can either print the thesis or read it on the customer computer screen.
  • You cannot save the thesis file on a flash drive or email it.
  • You cannot copy text or images from the file.
  • You cannot edit the file.

Printing the thesis

  • You can print the thesis for your personal study or research use.
  • Aalto University students and staff members may print black-and-white prints on the PrintingPoint devices when using the computer with personal Aalto username and password. Color printing is possible using the printer u90203-psc3, which is located near the customer service. Color printing is subject to a charge to Aalto University students and staff members.
  • Other customers can use the printer u90203-psc3. All printing is subject to a charge to non-University members.
Location:P1 Ark Aalto     | Archive
Keywords:transcriptome
rats
knockout
transgene
RNASeq
microarray
GSEA
Abstract (eng): The transcriptome represents the DNA in the form of mRNA and it encodes proteins.
Studying the transcriptome has always given scientists insights into the proteins synthesised in the various regions of the body and its functions.
This project deals with the computational analysis of the heart transcriptome of an over-expressing rat and a knockout rat, the outcome of which will shed light on the factors influencing the functioning of the heart.

The project was split into three parts.
The first two parts dealt with RNASeq analysis of the over-expressing rat where the transcriptome was subjected to differential expression analysis at the gene level and at the exon level respectively.
The outcome provided some interesting genes and exons at the top of the list.
The gene level analysis was extended to Gene Set Enrichment Analysis and a number of pathways were obtained.
The third part of the project dealt with micro array data analysis of knockout rats.

The final output showed the obvious differential expression of the knockout gene while the rest of the genes showed minimum variation under normal conditions.
Future directions for this project would involve further analysis on the biological aspects of the pathways obtained from the RNASeq data analysis to shed light on the impact of the transgene on them and unravelling other functionalities of the gene while also identifying new and unknown transcripts using tools like Cufflinks and annotating the exons.
ED:2012-09-19
INSSI record number: 45272
+ add basket
« previous | next »
INSSI